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shRNA design and construction

Service Introduction

        RNA interference (RNAi) is a powerful tool that has been widely used to identify and confirm RNAi targets. It can be achieved by synthesizing siRNA in vitro or constructing shRNA expression vectors. shRNA expression vectors can be directly used for cell transfection to perform RNAi operations, with more lasting inhibition effects. The resulting shRNA plasmid vectors can also be efficiently copied and used, making them a valuable tool for RNAi research.

Service Advantages

        Professional target design: We design target site sequences for specific genes. For each gene, we design 2-3 sequences to ensure that at least one of them can be successfully knocked down.

        Advanced technology: We have developed a fast and efficient shRNA cloning and construction method that is not affected by the hairpin structure, with a very high cloning and construction success rate.

        Reliable quality: The synthesized shRNA plasmids are verified by sequencing to ensure that the sequence is 100% correct, providing reliable sequencing results.

        Fast and efficient: The synthesis cycle is short, and the construction of shRNA with less than 5 sequences only takes 5-7 working days.

Technical Procedures

Target site design diagram

Services Provided

        shRNA design: We provide shRNA design and comprehensively analyze the optimal experimental scheme to facilitate the rapid and efficient progress of the experiment.

        Online query of experimental progress: We have professional staff to communicate with you in a timely and effective manner to address any problems that may arise during the project, and to ensure that the project is completed on schedule and with high quality.

        IP protection: We strictly protect the information provided by customers and will not disclose it to third parties in any form.

Results Delivery

Sample Requirements

        Self-provided vector: Please ensure that the provided vector is consistent with the relevant information.

        Plasmid: The plasmid should have a single, bright band, be dissolved in ddH2O, and be free of other impurities. The total amount should be approximately 1-2 μg.

        Bacterial culture: The bacterial culture should be fresh and free of contamination. The culture time should not be too long to prevent the bacteria from aging and causing plasmid extraction to fail.

Notes 

        Please provide the sequence of the gene to be silenced or the designed shRNA target site sequence.

        If you need to clone to a self-provided vector, please provide the relevant information about the vector (plasmid map, full sequence, multiple cloning site, validation results of the sequence upstream and downstream of the cloning site). If the vector has special requirements, please note when placing an order.